We present a list of the reptiles of the municipality of Vitória, Espírito Santo, Brazil, compiled through primary data (specimens gathered by the authors) and secondary data (specimens housed at museums and records available in literature). We record 51 reptile species distributed by the orders Crocodylia (one species), Testudines (nine species), and Squamata (forty-one species), subdivided in amphisbaenians (three species), lizards (eleven species), and snakes (twenty-seven species). We recorded six species that are listed as threatened in the Brazilian List of Endangered Species, as the terrestrial lizard Cnemidophorus nativo and the marine turtles Lepidochelys olivacea and Dermochelys coriacea.