Check List 20(4): 859-867, doi: 10.15560/20.4.859
Molecular methods confirm the first report of the non-indigenous Perna viridis Linnaeus, 1758 (Mytilida, Mytilidae) in southern Brazil
expand article infoMayara Carneiro Beltrão‡, Natalia Joana Rebello da Cunha§, Yan de Oliveira Laaf‡, Fernando Luiz Diehl§, Tayse Demetro dos Santos§
‡ University of Vale do Itajaí, Itajaí, Brazil§ Acquaplan Tecnologia e Consultoria Ambiental, Balneário Camboriú, Brazil

Corresponding author: Mayara Carneiro Beltrão ( mbeltrao@outlook.com )

Academic editor: Igor Christo Miyahira
© Mayara Carneiro Beltrão, Natalia Joana Rebello da Cunha, Yan de Oliveira Laaf, Fernando Luiz Diehl, Tayse Demetro dos Santos.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Beltrão MC, da Cunha NJR, Laaf YO, Diehl FL, dos Santos TD (2024) Molecular methods confirm the first report of the non-indigenous Perna viridis Linnaeus, 1758 (Mytilida, Mytilidae) in southern Brazil. Check List 20(4): 859-867. https://doi.org/10.15560/20.4.859
Open Access
Abstract
The mussel Perna viridis, commonly known as Green Mussel, is native from the Indo-Pacific region and has been introduced in various countries around the globe. In Brazil, the species has already been recorded in Rio de Janeiro and Ceará states. With the aim of assessing the presence of mussels in the southern region of the country, 14 individuals were collected in the Paranaguá Estuarine Complex, Paraná. The mussels were found attached at a depth of 2 m on the artificial structure of Ponta do Poço Marina. The DNA was extracted using a commercial kit, the COI gene was amplified through PCR using the primers dgL-CO-1490 and dgHCO-2198, sequenced by the Sanger method, and the species was identified through the BOLD Systems. The phylogenetic tree was built on the software MEGA 11 using 28 sequences from three Perna species. Therefore, the present study confirms the occurrence of P. viridis in Brazil through molecular identification for the first time and adds a second Brazilian state where it has been recorded. The Brazilian coastline provides optimal environmental conditions for the establishment and development of P. viridis. 
Keywords
DNA analysis, Green Mussel, invasive species, marine species, Paraná