First record of Boana maculateralis ( Caminer & Ron , 2014 ) and Boana tetete ( Caminer & Ron , 2014 ) ( Anura , Hylidae ) in Colombia

This is the first report demonstrating that Boana tetete and B. maculateralis occur in the Colombian Amazon Basin. These specimens were identified previously as Boana fasciata; however, morphology and current distribution records show that the specimens were misidentified. These new records extend the known distribution of the B. tetete 630 km north from nearest locality in the Ecuadorian Amazon and 420 km for B. maculateralis. An updated map of current distribution and ecoregional areas in Amazonian is provided.

The taxonomic status of populations in the calcarata-fasciata species complex in Colombia has not been evaluated; only 2 species are currently recognized: B. calcarata and B. fasciata (Acosta 2017).Four specimens of Boana deposited in reference collections of Amphibian collection of the Alexander von Humboldt Biological Resources Research Institute (IAvH-Am) were preliminarily identified as B. fasciata.However, diagnostic characters described in the taxonomic revision by Caminer and Ron (2014) indicate that those Colombian specimens are not that species.This revised identification is also consistent with the geographical distribution of B. fasciata, which is restricted to southern Ecuador and northern Peru (Caminer and Ron 2014).Morphological examination of the specimens indicate that they belong to B. tetete and B. maculateralis.Herein we document those records, which are the first known occurrences of both species for Colombia.

Methods
Specimens were collected using the active manual capture method along a terrestrial transect associated with riparian habitat.Specimens were euthanized using a solution of Clorethone and fixed in 10% formalin, then transferred and stored in 70% ethanol.Voucher specimens are deposited in the amphibian collection of the Alexander von Humboldt Biological Resources Research Institute (IAvH-Am), Villa de Leyva, Boyacá Department, Colombia.The specimens were identified using the original species' descriptions of the Boana calcaratafasciata species complex (Caminer and Ron 2014).Morphometric measurements were made with digital calipers (nearest 0.01 mm) for adult specimens following Caminer and Ron (2014).An updated occurrence map of B. tetete and B. maculateralis in the Amazonian region, based on collection data, was generated using Arc-GIS version 10.2.1.Specimens were collected under permits issued to the Instituto de Investigación de Recursos Biológicos Alexander von Humboldt (IAvH) (Decree 1376 of 2013).
This specimen is the first record of B. tetete in Colombia.The nearest previously known record is from San Jacinto, Loreto region, Peru (Caminer and Ron 2014), which is about 630 km away (Fig. 5).
Natural history.The specimen of B. tetete (IAvH-Am-14856, SVL = 33.6 mm, TD = 2.97 mm, TD/SVL = 0.08, Fig. 1) was collected during a rainy night between 21:00-22:00 h, the individual was found perched on shrubby vegetation about 1 m high on the margins of La Lupuna stream in a secondary forest associated with low hills.La Lupuna stream, a tributary of the Loretoyacu river (black water), is 1.5 m wide, 20 cm deep, and with clear waters (pH = 5.3; conductivity = 27uS/cm; total dissolved solids = 15 ppm; temperature = 29 °C).
These specimens represent the first records for Colombia of B. maculateralis from two distant localities in the Amazon basin (Fig. 5).The closest previously known records are from Río Napo, La Primavera sector, Orellana province, Ecuador (Caminer and Ron 2014), which are 420 km and 824 km from the Sierra of Macarena and Amacayacu National Natural Park, respectively (Fig. 5).
Identification.Boana maculateralis is a small frog (SVL 31.8-55.3mm) that can be distinguished from other species by the following set of morphological characters (Caminer and Ron 2014): calcar large and triangular (Fig. 2) (calcar absent, only small tubercle present in B. alfaroi and B. tetete; small and conical in B. fasciata); supernumerary tubercles on the hands not prominent or abundant (Fig. 3) (prominent and abundant in B. steinbachi); hidden surfaces of thighs (Fig. 4

Discussion
With this report of 2 additional species of Boana in Colombia, the number of species is increased to 23.Our finding of misidentified museum specimens points to the need to pursue exhaustive revision of material identified as B. calcarata and B. fasciata in Colombian collections, which will help better determine the geographic range of B. tetete and B. maculateralis.In Colombia, the Boana calcarata-fasciata species complex may also include B. alfaroi and other undescribed candidate species.
However, the lack of genetic data, live specimens, and vocalizations are some clear limitations when studying old museum specimens, and Caminer and Ron ( 2014) the exploration of candidate species in Colombian populations.
Boana tetete was originally described from 3 localities in Ecuador and Peru (Caminer and Ron 2014), with records located in the Tropical Moist Broadleaf Forest biome.In addition to this biome, ecoregional criteria (Olson et al. 2001) confirm that B. tetete occupies the Napo Moist Forests and Iquitos Varzeá ecoregions; there is the possibility that the range of this species extends to other ecoregions in the Amazonian basin (Fig. 5).
In contrast, B. maculateralis was previously reported from 24 localities in Ecuador and Peru (Caminer and Ron 2014).This species has a widespread distribution within the Amazon basin and was previously known from the Tropical Moist Broadleaf Forests biome and 3 ecoregions (Napo Moist Forests, Iquitos Varzeá, and Southwest Amazon Moist Forests).The record from Macarena extends this species' range into the Caquetá Moist Forests ecoregion (Fig. 5).
) and flanks with dark brown blotches (dark brown irregular spots in B. alfaroi and B. tetete; thighs with black transverse stripes in B. dentei sensu Bokermann 1967 and with dark brown vertical bars in B. calcarata and B. fasciata).