New records of Ephemeroptera ( Insecta ) from Tocantins state , northern Brazil

New records and notes on the distribution of the Ephemeroptera are presented. The genera Campylocia, Amanahyphes, Traverhyphes, Simothraulopsis, Tikuna, and Ulmeritoides are reported from Serra do Lajeado Environmental Protection Area, Tocantins state. With the results of the present study, the number of species known for Tocantins state has increased from two to seven.


INTRODUCTION
The order Ephemeroptera (Insecta) is considered an oligodiverse group, represented by about 3300 species distributed in 42 families (Barber-James et al. 2013).A recent checklist of Brazilian Ephemeroptera (mayflies) recorded 344 species from this country (Salles et al. 2016).
The ephemeropteran fauna is considered poorly known (less than four species recorded) for six of the 27 Brazilian states (Acre, Alagoas, Paraiba, Rio Grande do Norte, Sergipe, and Tocantins) as well as the Federal District (Salles et al. 2016).Only one genus, Camelobaetidius Demoulin, 1966 and  This study presents new records and distributional notes for mayflies from Tocantins, northern Brazil, based on collections from the Serra do Lajeado Environmental Protection Area.

MATERIALS AND METHODS
We collected ephemeropteran specimens from a riffle in a gallery forest (10° 13ʹ 50.93ʺ S, 048° 07ʹ 19.40ʺ W), from 21-25 March 2016, at the Evilson waterfall, district of Taquaruçu, in the Serra do Lajeado Environmental Protection Area (APA), Palmas, Tocantins state, Brazil (Figure 1).The distribution map was prepared using the software DIVA-GIS, version 7.5..The subimagos were captured with light traps from 18:00-21:00 h, and nymphs were captured with an aquatic entomological net.Male genital structures were examined in alcohol gel and then stored in alcohol.Permanent slides of nymph mouthparts, legs, and gills were prepared using Euparal® as the mounting medium.
The material examined is deposited in the Universidade Federal de Roraima Zoological Collection (UFRR), Boa Vista, RR, Brazil, and in the Universidade Federal do Tocantins Entomological Collec tion (CEUFT), Porto Nacional, Tocantins, Brazil.

RESULTS
The new records of mayfly species from Tocantins are proved below.For each species we list geographical distribution data and provide a diagnosis.

Family Leptophlebiidae
Simothraulopsis demerara (Traver, 1947) (Figures 5, 6) Diagnosis.Male imago: 1) Fork of MA of forewings asymmetrical, stem of MA straight; 2) costal projection of hind wings strongly developed, located about 2/3 distance from wing base to apex; 3) hind wing vein Sc ending at base of costal projection; 4) hind wing vein MP unforked; 5) tarsal claws of a pair dissimilar, one apically hooked and the other obtuse, pad-like; 6) penes divided in apical half with one ventrally directed spine on each lobe (Figure 6).

DISCUSSION
Our study records six genera and five species for the first time from the state of Tocantins.It increases the number of genera known to seven, and to seven the number of species known.The subimago of Traverhyphes could not be identified to species, and additional specimens are needed.

Figure 1 .
Figure 1.Map on the left shows the Brazilian states, highlighting the Tocantins state (gray) and the Palmas municipality (yellow-red point).The right map shows the hydrographic basin of the Brazilian Amazon (green) and the collections location (Evilson waterfall, yellow-red point).